Home FAQs When I thaw Isothermal Amplification Buffer II I see a lot of white precipitate, is this normal?

FAQ: When I thaw Isothermal Amplification Buffer II I see a lot of white precipitate, is this normal?

Yes. Due to the high concentration of salts (MgSO4, KCl) in 10X Isothermal Amplification Buffer II, there is commonly precipitation during freeze/thaw cycles. This will easily go back into solution with vortexing, and buffer should be thoroughly vortexed before use.