FAQ: What are the main causes of reaction failure using Bst 3.0 DNA Polymerase?

Temperatures below 50 °C or over 72 °C can significantly reduce enzyme activity. In addition, failure to use the appropriate buffer containing 50–150 mM KCl, 2–15 mM Mg, or pH outside of the range pH 7.5–10 cause reaction failure. See activity in other buffers above.