FAQ: Can Bst 3.0 DNA Polymerase be used to fill in 3' overhangs?

No, DNA polymerases cannot fill in 3' overhangs. To create blunt ends 3' overhangs must be removed. DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210), T4 DNA Polymerase (NEB# M0203) and Mung Bean Nuclease (NEB# M0250) are the best choices to remove 3' overhangs.