FAQ: Can Bst 3.0 DNA Polymerase be used to blunt DNA?

5' overhangs (3' recessed ends) can be filled in, but 3' overhangs will not be removed because the enzyme lacks an exonuclease activity. DNA Polymerase I, Large (Klenow) Fragment (NEB# M0210), T4 DNA Polymerase (NEB# M0203) and the Quick Blunting™ Kit (NEB #E1201) are the best choices for creating blunt DNA products.