FAQ: Can α1-3,4 Fucosidase and α1-2,3,4,6 Fucosidase cleave branched and complex substrates?

α1-3,4 Fucosidase and α1-2,3,4,6 Fucosidase can cleave simple branched substrates to completion in 1 hour. However, a higher enzyme concentration and a longer incubation time (4 hours to overnight) may be needed for complete digestion of complex substrates with multiple branches.