HomeFAQsWhy is the specific activity of the probe low?
FAQ: Why is the specific activity of the probe low?
The concentration of the radioactive nucleotide should be made limiting (6 μM). In other words, if using labeled ATP, add 500 μM of UTP, CTP, and GTP but only 6 μM ATP in the transcription so ATP is incorporated at higher percentage resulting very hot RNA probe.
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