Home FAQs Is it possible to start transcription with an A?

FAQ: Is it possible to start transcription with an A?

T7 RNA Polymerase can start transcription with an A under the class II T7 promoter. Similar to the regular T7 promoter, G is preferred at 2nd and 3rd position. 

T7 Promoter class II
5'- TAATACGACTCACTATTA 3'