Due to the acidity and lability of sulfate groups present on GAGs, ESI is the preferred technique because it can result in little or no in-source fragmentation when properly tuned (1). Optimization of the ESI tuning parameters is crucial for the accurate MS analysis of sulfated GAGs.
Extent and position of sulfation can lead to varying degrees of lability for every oligosaccharide. The lability of the sulfate groups increases as the size of the heparin oligosaccharide increases.
Successful ESI tuning parameters are more easily achieved using highly sulfated hexasaccharide standards with defined structures (NEB# P0738, P0739) as compared to smaller disaccharide standards
Kim, Y. S et al. (1998) Glycobiology 8(9):869-877. PMID: 9675219
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
