Magnetic Bead Based Methods

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Magnetic bead/particle-based purification methods also employ a bind-wash-elute process. However, instead of using centrifugation or vacuum manifolds to remove the aqueous phase from contact with the silica matrix, these workflows use magnetic beads or particles functionalized with silica surfaces to allow selective binding of DNA in the presence of high concentrations of salt. DNA bound to a magnetic bead can be easily separated from the aqueous phase using a magnet; thereby allowing rapid sample processing and fine control of solution volumes. Magnetic-based methods are ideal for automation of high throughput processing, as they eliminate the need for centrifugation and other time-consuming steps.