New England Biolabs® Introduces Novel Cloning and DNA Assembly Method: NEBuilder® HiFi

NEBuilder HiFi allows for DNA assembly with lower input requirements and increased fidelity, without requiring any licensing fees from NEB

Ipswich, MA (January 30, 2015) -- New England Biolabs (NEB®) announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes. The novel, proprietary DNA polymerase in the NEBuilder HiFi enzyme mix results in extremely accurate fragment assemblies and cloning junctions, and can assemble DNA fragments ranging from 100 bp to at least 19 kb. The unique enzyme formulation of NEBuilder HiFi, which has numerous benefits over the Gibson Assembly® Master Mix (also available from NEB), further allows the joining of DNA fragments by use of a synthetic oligonucleotide splint. Experimental data highlighting the superior performance and quality of NEBuilder HiFi is available at In addition, no licensing fees from NEB are required for commercial organizations using NEBuilder HiFi.

“NEB has supplied DNA assembly reagents for over 40 years and the new NEBuilder HiFi DNA Assembly Master Mix is the result of our commitment to producing high quality, innovative cloning reagents. The same reagent allows fast, virtually error-free standard cloning, as well as large, complex multi-fragment DNA assemblies. We continue to find new applications in which it excels and saves us valuable research time,” says Tom Evans, Ph.D., who leads the DNA Enzymes Division at NEB.

NEB also provides a free tool, called the NEBuilder® Assembly Tool (, to simplify the design of primers. Scientists using fragments designed using other seamless cloning methods can easily assemble these with NEBuilder HiFi, due to compatibility of the fragments.

About New England Biolabs

Established in the mid 1970's, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK. For more information about New England Biolabs visit

NEB®, NEW ENGLAND BIOLABS®, and NEBUILDER® are registered trademarks of New England Biolabs, Inc.

GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.

Contact information:
Deana D. Martin, Ph.D.
Marketing Communications Manager
New England Biolabs
240 County Road
Ipswich, MA 01938
Tel: 978-380-7464


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