RNA Capping Enzymes' Properties Chart
Please click here to select capping enzymes by application.
| NEB Product |
Catalog # | Substrate | Reaction Products | Buffer/ Co-substrates | Incubation activity range (preferred) |
|---|---|---|---|---|---|
| RNA Capping Enzymes | |||||
| Faustovirus Capping Enzyme (FCE) | M0281 | 5´ ppp-RNA, 5´ pp-RNA | m7GpppN- RNA (Cap-0) |
Capping Buffer, SAM (methyl donor), GTP (cap donor) |
25-50°C (37°C) |
| Vaccinia Capping System (VCE) | M2080 | 5´ ppp-RNA, 5´ pp-RNA | m7GpppN- RNA (Cap-0) |
Capping Buffer, SAM (methyl donor), GTP (cap donor) | 30-45°C (37°C) |
| 5´ Cap Modifications | |||||
| mRNA Cap 2´-O-Methyltransferase | M0366 | m7Gppp N- RNA (Cap-0) | m7GpppNm- RNA (Cap-1) | Capping Buffer, SAM (methyl donor) |
25-37°C (37°C) |
| mRNA Cap N6 Methyltransferase (m6A) | M0767 | m7Gppp A- RNA (Cap-0) m7Gppp Am- RNA (Cap-1) |
m6A Cap-0 m6Am Cap-1 |
Capping Buffer, SAM (methyl donor) |
25-40°C (37°C) |
