NEB Scientific Posters

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  1. Reproducible performance, versatility, and point-of-use optimization of NEBExpress® Cell-free E. coli Protein Synthesis System

    Systematic evaluation of the NEBExpress® Cell-Free E. coli Protein Synthesis System demonstrates reproducible, versatile, and high-yield protein expression across diverse targets, templates, and workflows.

  2. Cold-Active TEV Protease: Engineered for higher performance at low temperatures

    Cold-Active TEV Protease has been engineered specifically for higher activity at lower temperatures, offering both increased cleavage speed and protection of protein integrity.

  3. NEBNext enzymatic solutions for challenging samples & workflows

    Improvements in library quality and variant calling accuracy of NEBNext UltraShear® and NEBNext UltraShear FFPE DNA Library Prep to FFPE Samples in a hybrid capture workflow.

  4. NEBNext UltraShear® Long Read: Novel and time-dependent enzymatic DNA fragmentation for long read sequencing

    Novel solution for enzymatic fragmentation for long-read sequencing.

  5. Optimized NEBNext UltraExpress® workflows enable high-performance RNA and DNA library construction from low inputs

    Application and performance recommendations of NEBNext UltraExpress DNA and RNA for low-input samples.

  6. Accurate representation of small RNAs using NEBNext Low-bias Small RNA Library Prep Kit

    Novel single-day ligation-based small RNA library preparation method demonstrates reduced bias, enhanced sensitivity, and accuracy of sncRNA detection.

  7. Enzymatic Methyl-seq enables simultaneous analysis of methylation and germline variants from whole genome and target capture data

    NEBNext® Enzymatic Methyl-seq generates consistent and accurate methylation calls using various sample types across a wide range of inputs. The use of base conversion in EM-seq™ poses a challenge to variant detection, however, this can be resolved bioinformatically because methylation information is preserved on only one strand in EM-seq libraries, while the other strand can be used to detect genetic variation. Here we present a bioinformatic workflow that demonstrates the ability to call germline SNPs with high recall and precision. With this ability to assess methylation state and genetic mutations from a single library we can maximize the utility of our sequencing datasets.

  8. NEBNext® enzymatic solutions for DNA methylation profiling of highly damaged DNA inputs

    Fragmented or low-quality DNA, including FFPE DNA, can be challenging to analyze for epigenetic marks, but new techniques demonstrate reliably high-quality methyl-sequencing profiles. Enzymatic fragmentation, when used upstream of enzymatic methylation mapping, improves library data quality metrics as compared to mechanical shearing with Covaris.

  9. A Sample-to-results Workflow for Agnostic or Targeted Pathogen Sequencing from Wastewater

    Population-level pathogen surveillance of wastewater can identify disease outbreaks early. In this poster, we present a workflow for detecting the example pathogens, influenza A (flu A) and respiratory syncytial virus (RSV), with NEBNext library prep in concert with Nanotrap Microbiome A and B particles.

  10. New single day EM-seq and E5hmC-seq workflows: Faster library prep for whole genome and target enrichment methylome profiling

    In this poster, we demonstrate a faster, single-day workflow for NEBNext Enzymatic Methyl-seq v2 and NEBNext Enzymatic 5hmC-seq, featuring a reduced deamination time.

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