Home FAQs When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), why is there a gel-like pellet in the column after the first elution?

FAQ: When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), why is there a gel-like pellet in the column after the first elution?

A gel-like pellet may be visible in the column after the first elution. This contains RNA of interest that is not fully resuspended in the nuclease-free water. It is recommended to perform additional elution steps. A second elution (or third elution in the case of RNA sizes ≥ 9 kb) will recover remaining RNA of interest.