HomeFAQsWhat size DNA can be purified with the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135)?
FAQ: What size DNA can be purified with the Monarch® Spin High-Capacity DNA Cleanup Kit (NEB #T1135)?
Recovery of DNA from 50 bp to 25 kb can be accomplished with the Monarch Spin High-Capacity DNA Cleanup Kit (100 µg) (NEB #T1135). Efficiency decreases with longer DNA due to tighter binding to the matrix. The recovery of DNA ≥ 15 kb can be improved by modifying the elution method, using heated elution buffer (50°C) and extending incubation times. For optimal recovery of large DNA fragments, please follow the instructions in the manual. For DNA > 25 kb, we provide a modified protocol optimized for large DNA on our webpage. Please refer to the protocol available on webpage for detailed procedural guidance.
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