FAQ: What is the smallest volume of elution buffer that can be used with the Monarch® Spin Columns S3A (NEB #T1157)?

The range for elution volume using Monarch Spin Columns S3A with Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) is 50 - 200 μl, with 100 μl as a recommended elution volume. When using minimum elution volume, it is recommended to incubate at room temperature for a few more minutes than recommended to maximize yield.