FAQ: Can I use water to elute the DNA when using the Monarch Spin Plasmid Miniprep kit?

Yes, water can be used to elute DNA from Monarch columns. For maximum elution efficiency, ensure the water is nuclease-free and the pH is between 7-8.5. Milli-Q™ water is often slightly acidic, requiring pH adjustment. If you are storing the DNA long-term, we recommend using the supplied DNA elution buffer, which contains 0.1 mM EDTA and can help inhibit metal-dependent nucleases.