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Cas Nucleases Selection Chart

Use this chart and key to select from our Cas9 and Cas12 nuclease proteins by their recommended applications, including RNP delivery, in vitro target detection assays, visualization and cloning.

 

Cas9 Nuclease, S. pyogenes

EnGen® Lba Cas12a (Cpf1)

EnGen Sau Cas9

EnGen Seq1 Cas9

EnGen Spy Cas9 HF1

EnGen SpRY Cas9

EnGen Spy Cas9 Nickase

EnGen Spy Cas9 NLS

EnGen Spy dCas9 (SNAP-tag®)

GENOME EDITING APPLICATIONS

In vitro digestion of DNA

+++

+(x)

++

++

++

+++

++(y)

++

  

Transfection of RNP directly into cells

+

+++

+++

+++

+++

  

+++

+++

+++

Disruption of a gene of interest via NHEJ or HDR application

+

+++

+++

+++

+++

    

+++

  

High-fidelity genome editing

        

+++

        

Longer PAM for reduced off-target effects

  

+++

+++

++

    

++(y)

    

Programmable site-specific DNA nicking for genome editing and in vitro applications

            

+++

    

Wide temperature range for enzyme activity

  

+++

  

+++

          

Visualization and target enrichment via attachment of fluorophores, biotin, and several other conjugates.

                

+++

Target AT-rich regions

  

+++

+++

            

Collateral Cleavage (trans cleavage) activity and
Target Detection

  

+++

              

Contains NLS (nuclear localization signal)

  

+++

+++

+++

+++

++

+++

+++

+++

Key

+++

Optimal, recommended nuclease for this application

++

Works well for the selected application

+

(x)

Will perform selected application, but it is not recommended

Has trans nuclease activity

(y)

When using two RNA guides, one targeting each strand.