HomeFAQsWhy was the low-speed spin (1,000 x g for 3 minutes) removed from the protocols in the Monarch Spin gDNA Extraction Kit (NEB #T3010)? Can I still perform this low-speed spin?
FAQ: Why was the low-speed spin (1,000 x g for 3 minutes) removed from the protocols in the Monarch Spin gDNA Extraction Kit (NEB #T3010)? Can I still perform this low-speed spin?
The protocol change to remove the low-speed spin step (1,000 x g for 3 minutes) in the binding step is for streamlining the workflow and saving time. Our testing has shown no impact on performance or yield. Kits using orange-tinted Monarch Spin Columns S2C (NEB #T3017) are encouraged to follow the updated protocols for improved efficiency.
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