Home FAQs What should I do if my RNA does not have 5' monophosphate and 3' OH ends before starting the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?

FAQ: What should I do if my RNA does not have 5' monophosphate and 3' OH ends before starting the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?

T4 PNK can add 5' monophosphate to a 5' OH and remove 3' phosphate generating a 3' OH. Other modifications such as caps or pyrophosphates would need to be removed with other enzymes, contact NEB technical support (info@neb.com) for recommendations.