The main substrates of TDG are dU or dT mismatched with dG in duplex DNA, with a preference for a CpG context (1). In addition, TDG has been reported to have a wide substrate tolerance, including activity on 5-formylcytosine and 5-carboxylcytosine (oxidation products of 5mC)(2), 5-hydroxymethyluracil(3), 3, N4- ethenocytosine(4), 5-fluorouracil(5), and other modified derivatives(6).
References:
1. Waters, T.R. and Swann, P.F. (1998). J Biol Chem. 273, 20007-14.
2. He Y.F. et al. Science. (2011). 333, 1303-07.
3. Baker D. et al. (2002) Chem Res Toxicol. 15, 33-9.
4. Saparbaev, M. and Laval, J. (1998). PNAS. 95, 8508-13.
5. Hardeland, U. et al. (2000). J Biol Chem. 275, 33449-33456.
6. Bennett M.T. et al. (2006). J Am Chem Soc. 128(38), 12510-9.
