Home FAQs Are libraries generated with the NEBNext Low-bias Small RNA Library Prep Kit compatible with paired-end Illumina sequencing?

FAQ: Are libraries generated with the NEBNext Low-bias Small RNA Library Prep Kit compatible with paired-end Illumina sequencing?

Yes, libraries prepared with this protocol can be sequenced with paired-end reads. However, due to the small insert sizes, a single-end read of 56 bp is usually sufficient, and a second read would be redundant.