RNA 5´ Modification Enzyme Properties Chart
Please click here to select enzymes that modify 5’ RNA ends by application.
| NEB Product |
Catalog # | Substrate | Reaction Products | Buffer/ Co-substrates | Incubation activity range (preferred) |
|---|---|---|---|---|---|
| mRNA Decapping | |||||
| mRNA Decapping Enzyme | M0608 | m7Gppp-RNA cap (Cap-0 or Cap-1) | 5´ p- RNA, m7GDP | 100% activity in mRNA Decapping Enzyme Reaction Buffer. 50% activity in rCutSmart, NEBuffer r1.1, ThermoPol Buffers. | 37-45°C (37°C) |
| yDcpS | M0643 | m7Gppp-RNA cap (Cap-0 or Cap-1) | 5´ pp- RNA, m7GMP | yDcpS Reaction Buffer | 37-50°C (37°C) |
| NudC Pyrophosphatase | M0607 | NAD+/NADH caps | 5´ p- RNA, NMN | NEBuffer r3.1 | 37°C |
| Generating Ligatable 5´ Ends | |||||
| RNA 5´ Pyrophosphohydrolase (RppH) | M0356 | 5´ ppp- (primary) | 5´ p- RNA | NEBuffer 2 (pyrophosphatase) | 37°C |
| Capped RNA (secondary) | ThermoPol (decapping) | ||||
| Apyrase | M0398 | 5´ ppp-RNA | 5´ p-RNA | 100% activity in Apyrase Reaction Buffer. 30% activity in all NEBuffers. | 30°C |
| ATP | AMP | ||||
| 5´ DNA Adenylation Kit | E2610 | 5´ p- ssRNA | 5´ App- ssRNA | 5´ DNA Adenylation Reaction Buffer | 37-65°C (65°C) |
| 5´ p- ssDNA | 5´ App- ssDNA | ||||
| 5´ Deadenylase | M0331 | 5´ App- RNA | 5´ p- ssRNA | NEBuffer1 | 30°C |
| 5´ App- DNA | 5´ p- ssDNA | ||||
| T4 Polynucleotide Kinase | M0201 | 5´ OH-RNA, RNA-p 3', RNA-2',3' cyclic phosphate | 5´ p-RNA-OH 3' | T4 Polynucleotide Kinase Reaction Buffer. rCutSmart (+ ATP, DTT). | 37°C |
| 5´ OH-DNA, DNA-p 3' | 5´ p-DNA-OH 3' | ||||
| 5´ Phosphatases | |||||
| Quick CIP (recommended) | M0525 | 5´ ppp- RNA, 5´ pp- RNA, 5´ p- RNA | 5´ OH- RNA | rCutSmart, other NEBuffers | 37°C |
| 5´ ppp- DNA, 5´ pp- DNA, 5´ p- DNA | 5´ OH- DNA | ||||
| NTP, NDP, dNTP | Nucleoside | ||||
| Antarctic Phosphatase | M0289 | 5´ ppp- RNA, 5´ pp- RNA, 5´ p- RNA | 5´ OH- RNA | Antarctic Phosphatase Reaction Buffer. This enzyme requires zinc. | 37°C |
| 5´ ppp- DNA, 5´ pp- DNA, 5´ p- DNA | 5´ OH- DNA | ||||
| NTP, NDP, dNTP | Nucleoside | ||||
