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Cell-free DNA Synthesis for a Faster Path to RNA and DNA Therapeutics
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8 tips to Follow When Choosing a Restriction Enzyme for In vitro mRNA Transcription for Vaccine and Therapeutics Production
Originally published in 2021; updated in March 2026
Choosing the right restriction enzyme is a critical first step in in vitro transcription for mRNA vaccine and therapeutic production. This post discusses key factors that influence enzyme choice and explains how careful selection supports efficient IVT, reduces unwanted by‑products, and enables scalable, compliance‑ready mRNA manufacturing.Topic:
A Universal PCR Annealing Temperature Means Balancing Convenience with Specificity
Annealing temperature is a critical parameter that can determine PCR specificity and yield. Some workflows benefit from a single thermocycling protocol across numerous primer sets. NEB scientists evaluated the benefits and limitations of using a universal annealing temperature to achieve robust amplification across diverse targets.
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What to Do After Your Life Science Degree: Post-baccalaureate and Post-doctoral Programs
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Building Genes Faster and Cheaper with Decentralized Workflows
Read about a new decentralized workflow from NEB researchers that’s redefining how scientists synthesize genes. By combining data-optimized design with advanced assembly techniques, this approach delivers sequence-verified constructs in as little as four days—at a fraction of the traditional cost. With faster turnaround, higher fidelity, and fewer external dependencies, this workflow enables researchers to conduct complex gene synthesis projects more efficiently and at greater scale, supporting broader access to advanced synthetic biology methods.
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