Home FAQs When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), can I reload the eluate from the first elution on to the column for a more concentrated final product?

FAQ: When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), can I reload the eluate from the first elution on to the column for a more concentrated final product?

Yes, the eluate can be reloaded on to the column for a second elution. However, there may be some loss of yield. For RNA sizes ≥ 5 kb at 3 mg RNA input, a second elution with a fresh aliquot of nuclease-free water will produce the highest yield.