Home FAQs What is the maximum binding capacity of the Monarch® Spin Columns S3A provided with the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)?

FAQ: What is the maximum binding capacity of the Monarch® Spin Columns S3A provided with the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) (NEB #T1135)?

The matrix supplied in each column can bind up to 100 μg of DNA. Purifying DNA from a PCR or other enzymatic reaction introduces components such as detergents, dyes, proteins, and nucleotides, all of which can compete for binding to the membrane and affect DNA recovery.