HomeFAQsHow do I prepare a transposome using TnX® Transposase (Unloaded) (NEB #M2623)?
FAQ: How do I prepare a transposome using TnX® Transposase (Unloaded) (NEB #M2623)?
TnX Transposase (Unloaded) can be combined with TnX Diluent (supplied) and annealed adaptor oligonucleotides containing mosaic-end (ME) sequences (not supplied) to generate a transposome. Once assembled, the transposome can be used to simultaneously fragment double-stranded nucleic acids and insert adaptor sequences during tagmentation. Refer to the product protocol for recommended adaptor designs and transposome assembly conditions.
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