Home FAQs How can I scale up my adenylation reaction?

FAQ: How can I scale up my adenylation reaction?

For larger-scale applications, you can increase the reaction volume and use our High Concentration Mth RNA Ligase, available exclusively through the Custom Solutions channel. This allows the adenylation reaction to be scaled up to 30 pmol of oligonucleotide and 30 pmol of enzyme per µl without loss of efficiency. After the reaction, the oligonucleotide can be purified by phenol extraction and alcohol precipitation or column chromatography to effectively remove protein and ATP.