Home FAQs How are libraries prepared from cell-free DNA (cfDNA) using the NEBNext® EM-seq Kit (NEB #E7120)

FAQ: How are libraries prepared from cell-free DNA (cfDNA) using the NEBNext® EM-seq Kit (NEB #E7120)?

With cfDNA, there is no need to shear the DNA before library preparation. 10 – 200 ng of cfDNA can move directly into NEBNext Ultra II DNA library preparation, and then EM-seq enzymatic conversion and library amplification steps are followed as outlined in the product manual.