HomeFAQsCan I use water to elute the DNA when using Monarch® Mag PCR & DNA Cleanup Kits (5 μg) (NEB #T4130)?
FAQ: Can I use water to elute the DNA when using Monarch® Mag PCR & DNA Cleanup Kits (5 μg) (NEB #T4130)?
Yes, water can be used to elute the DNA. For maximum elution efficiency, ensure the water is nuclease-free and the pH is between 7 and 8.5. Milli-Q™ water is often slightly acidic, requiring pH adjustment. If you are storing the DNA long-term, we recommend using the supplied Monarch Buffer EY (DNA elution buffer), containing 0.1 mM EDTA.
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