Differences between electrocompetent and chemically competent cells

Learn how electrocompetent cells and chemically competent cells differ in the way uptake of DNA is achieved.

Script

Customer often ask, "What are the differences between electrocompetent and chemically competent cells?" These cells differ in the way uptake of DNA is achieved. Chemically competent cells are treated with a salt solution to allow bound DNA to travel through the membrane. And heat shock is used to further enhance DNA passage. Electrocompetent cells do not require DNA binding. Instead, an electrical field is used to allow DNA to pass through the membrane. And please remember, if you ever need any help you can always contact our technical support at info@neb.com.

Related Videos

  • NEBTV_CompetentCells_720
    Should I choose chemically competent or electrocompetent cells?
  • GlassBeadsvideo_1920
    Fast transformation plating with glass beads
  • TransformationTips_VideoThumb_282x210_0616
    Tips for Successful Transformations with NEB® Competent Cells
Visit NEB’s Video Library
Loading Spinner