RNA 5' Modification Enzymes' Selection Chart by Application
Please click here for properties of enzymes that modify 5’ RNA ends.
| NEB Product | Catalog # | Applications | Incubation activity range (preferred) | Tag |
|---|---|---|---|---|
| mRNA Decapping | ||||
| mRNA Decapping Enzyme | M0608 | Decapping G caps for ligation | 37-45°C (37°C) | His |
| yDcpS | M0463 | Decapping G caps for recapping with FCE/VCE. ReCappable Seq. |
37-50°C (37°C) | His |
| NudC Pyrophosphatase | M0607 | Decapping non-canonical capped RNA initiating with adenosine for ligation | 37°C | His |
| Generating Ligatable 5´ Ends | ||||
| RNA 5´ Pyrophosphohydrolase (RppH) | M0356 | Diphosphate removal of IVT RNA for ligation. Generating 5´ monophosphate on capped and uncapped RNA for XRN-1 digestion. Cappable Seq. |
37°C | None |
| Apyrase | M0398 | Generating 5´ monophosphate on IVT RNA for ligation. Generating 5´ monophosphate on capped and uncapped RNA for XRN-1 digestion. |
30°C | None |
| 5´ DNA Adenylation Kit | E2610 | Increase ssRNA ligation specificity with truncated T4 Rnl mutants. Structured or difficult ssRNA ligation with Thermostable 5´ App DNA/RNA Ligase. |
37-65°C (65°C) | His |
| 5´ Deadenylase | M0331 | Resolve abortive ligation intermediates (App-RNA, App-DNA) | 30°C | None |
| 5´ Phosphatases | ||||
| Quick CIP (recommended) | M0525 | Complete, iterative 5´ phosphate removal | 37°C | None |
| Antarctic Phosphatase | M0289 | Resolve abortive ligation intermediates (App-RNA, App-DNA) | 37°C | None |
