NEBNext® DNA library prep kits offer exceptional yields of high-quality libraries
Choose NEBNext for your DNA library preparation
• NEBNext offers multiple DNA library prep options for high yields and higher quality libraries upstream of sequencing.
• NEBNext UltraExpress™ DNA Library Prep Kit rapidly generates high-quality DNA libraries in under 2 hours with a single protocol for all inputs (10 ng to 200 ng).
• NEBNext Ultra™ II DNA Library Prep Kit for Illumina® generates high-quality DNA libraries from challenging samples and low input amounts (500 pg to 1 µg).
• Less hands-on time, and automation friendly
• Less consumables and fewer clean-up steps
Streamlined for Speed
NEBNext Ultra II DNA Library Prep Protocol Video
This video walks you through DNA library preparation using the NEBNext Ultra II DNA Library Prep Kit, including tips for optimization and safe stopping points.
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NEBNext for DNA Sample Prep Brochure
This brochure provides an overview of NEBNext sample preparation products for sequencing on the Illumina platform, as well as tech tips and workflow overviews.
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Protocol for use with NEBNext Ultra II DNA Library Prep with Sample Purification Beads
Comprehensive protocol for DNA library preparation for 500 pg to 1 μg fragmented DNA. Includes end prep, adaptor ligation, size selection, PCR amplification and cleanup.
Featured products
Discover the efficiency and quality of NEBNext DNA library preparation kits, tailored for diverse sequencing requirements. We offer innovative solutions like NEBNext UltraExpress and NEBNext Ultra II kits, ensuring high yields and superior library quality for your research needs.
NEBNext UltraExpress DNA Library Prep Kit
NEBNext UltraExpress DNA Library Prep Kit
This kit offers a fast (< 2 hour), streamlined workflow, from a broad DNA input range, while generating less plastic waste and a single protocol for all input amounts.
NEBNext UltraExpress FS DNA Library Prep Kit
NEBNext UltraExpress FS DNA Library Prep Kit
This kit includes FS enzymatic fragmentation in a fast (<2hrs), streamlined workflow, yielding high-quality libraries from a broad DNA input range, reducing plastic waste, and using a single protocol for all input amounts.
NEBNext Ultra II DNA Library Prep Kit for Illumina
NEBNext Ultra II DNA Library Prep Kit for Illumina
Generate high-quality libraries from a broad range of input amounts (from 500 pg to 1 μg) including challenging samples. This kit is available with
(NEB #E7103) and without
(NEB #E7645) sample purification beads.
NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina
NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina
This kit offers an amplification-free workflow for DNA library prep based on the streamlined and reliable Ultra II FS workflow from as little as 50 ng of DNA. Available with (NEB #E7435) and without (NEB #E7430) sample purification beads.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
