Home FAQs When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), why is there a pellet in the spin column?

FAQ: When using the Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) (NEB #T2060), why is there a pellet in the spin column?

A pellet containing the RNA of interest forms on the side of the column and on the O-ring following the first binding spin. Continue with the cleanup protocol for high-quality RNA in the eluate.