Home FAQs When is the RT primer annealed during the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?

FAQ: When is the RT primer annealed during the NEBNext Low-bias Small RNA Library Prep workflow (NEB #E3420)?

There is no separate RT primer in the NEBNext Low-bias Small RNA Library Prep Kit. The RT primer is generated from the bottom strand of the NEBNext LB 3' Adaptor during the NEBNext LB 5' Adaptor Ligation reaction step.