Home FAQs What are typical yields for a NEBNext Low-bias Small RNA library (NEB #E3420)?

FAQ: What are typical yields for a NEBNext Low-bias Small RNA library (NEB #E3420)?

In general, commercially available total RNA from human brain (RIN > 7; 1000 ng; 9 PCR cycles) brought through Section 8A of the Product Manual (Size Selection) can yield 30 – 60 nM final libraries, while the same input brought through Section 8B of the Product Manual (Cleanup) can yield 60 – 120 nM final libraries. Factors such as sample, small RNA content, RNA integrity, and PCR cycle number will impact final library yields.