If you cannot domesticate your sequence of interest to remove an XbaI site, you may use one of the alternative processing restriction enzymes optimized in the dbDNA™ vector backbone sequence: ApaLI, AseI, BamHI-HF, ClaI, FspI, NdeI, PacI, RsrII, SbfI-HF. Refer to the table below:
Restriction endonuclease information for downstream processing (removal) of dbDNA Vector backbone
| RESTRICTION ENZYME | CUTSITE |
NUMBER OF SITES
|
|---|---|---|
| ApaLI | 5´…G▼TGCAC…3´ 3´…CACGT▲G…5´ |
4 |
| AseI | 5´…AT▼TAAT…3 3´…TAAT▲TA…5´ |
4 |
| BamHI-HF® | 5´…G▼GATCC…3 3´…CCTAG▲G…5´ |
4 |
| ClaI | 5´…AT▼CGAT…3 3´…TAGC▲TA…5´ |
3 |
| FspI | 5´…TGC▼GCA…3 3´…ACG▲CGT…5´ |
4 |
| NdeI | 5´…CA▼TATG…3 3´…GTAT▲AC…5´ |
3 |
| PacI | 5´…TTAAT▼TAA…3 3´…AAT▲TAATT…5´ |
3 |
| RsrII | 5´…CG▼GWCCG…3 3´…GCCWG▲GC…5´ |
4 |
| SbfI-HF | 5´…CCTGCA▼GG…3 3´…GG▲ACGTCC…5´ |
3 |
| XbaI* | 5´…T▼CTAGA…3 3´…AGATC▲T…5´ |
3 |
* Supplied with EnClose™ Cell-free dbDNA™ Synthesis Kit.
