Home FAQs Can Cold-Active TEV Protease (NEB #P8118) cleave a fusion protein bound to an affinity column?

FAQ: Can Cold-Active TEV Protease (NEB #P8118) cleave a fusion protein bound to an affinity column?

While we have successfully demonstrated that Cold-Active TEV Protease can cleave a tag from a tagged protein immobilized on an affinity resin, we recommend testing the cleavage reaction on the fusion protein eluted from the affinity column first to determine the best conditions.