RNA Synthesis with HiScribe® T7
Choose HiScribe® for your RNA synthesis needs
Get high-quality RNA for therapeutics, research, or diagnostics with NEB’s trusted HiScribe® kits—optimized for flexibility, scale, and performance.
• High-yield synthesis of capped, poly(A)-tailed, or modified RNA
• Ideal for mRNA therapeutics, vaccine research and CRISPR
• Flexible template and cap analog compatibility for customization
Listen Up!
The message is spreading.
NEB has everything you need for your RNA-related workflows.
Scaling of High-Yield in vitro Transcription Reactions for Linear Increase of RNA Production
Recommendations for scaling workflows using HiScribe® T7/SP6 RNA Polymerase kits and individual enzymes (T7 RNA Polymerase, Pyrophosphatase, RNase Inhibitor).
Read Technical Note
RNA Synthesis Brochure
Learn more about NEB's products for synthesis of RNA, which range from template generation to poly(A) tailing.
View Brochure
Minding your caps and Poly A tails – Strategies for synthesizing in vitro transcribed (IVT) mRNA
Understand options for selecting reagents and the extent of reagent influence on synthesized mRNA cap functionality.
Read ArticleFeatured HiScribe Products for RNA Synthesis
Streamline your RNA synthesis with HiScribe—a versatile suite of high-yield kits for uncapped, capped, and polyadenylated RNA. From basic transcription to advanced mRNA workflows, these tools offer reliable performance and clean results. Capping enzymes and reagents are also available separately to fit your needs. Designed with sustainability in mind, HiScribe products minimize plastic use and feature recyclable packaging.
HiScribe® T7 High Yield RNA Synthesis Kit
HiScribe® T7 High Yield RNA Synthesis Kit
High-yield kit for in vitro transcription; produces up to 180 μg RNA and supports modified NTP incorporation for flexible applications.
HiScribe® T7 mRNA Kit with CleanCap® Reagent AG
HiScribe® T7 mRNA Kit with CleanCap® Reagent AG
Efficiently generates up to 90 µg of Cap-1 mRNA per reaction; supports modified NTPs and includes purification reagents.
Faustovirus Capping Enzyme
Faustovirus Capping Enzyme
Adds Cap-0 to RNA with high efficiency, broad temperature compatibility, and enables one-pot Cap-1 synthesis with minimal optimization.
N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)
N1-Methyl-Pseudouridine-5´-Triphosphate (N1-Methyl-Pseudo-UTP)
This modified NTP, supplied as a sodium salt at pH 7.5, reduces RNA immunogenicity, has ≥95% purity. NEB offers various modified nucleotides for RNA synthesis.
This content is covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
