Additional E. coli Strain Genotypes
These E. coli strains are not supplied by New England Biolabs. Sources are listed in orange type.
Strain Sources
A = ATCC (https://www.atcc.org),
CGSC = E. coli Genetic Stock Center (https://cgsc.biology.yale.edu),
LTI = Invitrogen Life Technologies, N = Novagen, S = Stratagene
| Strain (Source) | Ref | Genotype |
|---|---|---|
| 71-18 | 2 | F´ laclqΔ(lacZ)M15 proA+B+ /Δ(lac-proAB) thi glnV |
| BHB2688 (A) | 3 | F– recA λr (λEam 4 b2 red3 imm434 cIts Sam7) |
| BHB2690 (A) | 3 | F–ompT gal [dcm] [lon] hsdSB (rB– mB–; an E. coli B strain) with DE3, a λ prophage carrying the T7 RNA polymerase gene |
| BL21(DE3) (N) | 17 | BNN93 hflA150::Tn10 (TetR); The strain known as C600hfl is better known as BNN102 |
| BNN93 (A) | 4, 5, 6 | F– e14– (McrA–) hsdR (rK– mK–) glnV44 thr-1 leuB6 thi-1 lacY1 fhuA21 mcrB; Some isolates circulating as C600 are actually BNN93 |
| BNN102 (A) | 4, 5, 6 | BNN93 hflA150::Tn10 (TetR); The strain known as C600hfl is better known as BNN102 |
| C600 (CGSC) | 4, 5, 6 | F– [e14– (McrA–) or e14+ (McrA+)] thr-1 leuB6 thi-1 lacY1 glnV44 rfbD1 fhuA21; The original C600 is EcoK r+m+ McrBC+; See BNN93† |
| C600hfl | 4, 5, 6 | BNN102 is sometimes called C600hfl |
| CES200 (A, CGSC) | 1 | F– thr-1 ara-14 Δ(gpt-proA)62 lacY1 tsx33glnV44galK2hisG4rfbD1rpsL31 (StrR) kdgD51 xyl-5 mtl-1 argE3 leuB6 hsdR (rK– mK+) recB21 recC22 sbcB15 sbcC |
| CSH18 | 9 | F´Δ(lacZ)H125 proA+B+ /Δ(lac-pro)glnV thi |
| DB1316 (A, CGSC) | 1, 6 | F– recD1014 mcrB1 hsdR2 (rK– mK+) zjj202::Tn10 (TetR) |
| DH1 (LTI) | 8 | F–glnV44 recA1 endA1 gyrA96 (NalR) thi1 hsdR17 ((rK– mK+) relA1 spoT1? rfbD1? |
| DH5αF´ (LTI) | 6, 7 | F´/endA1 hsdR17 (rK– mK+) glnV44 thi-1 recA1 gyrA (NalR) relA1 Δ(lacIZYA-argF)U169 deoR (φ80dlacΔ(lacZ)M15) |
| DL538 | 6, 10 | NM621 sbcC201 |
| DP50 (A) | 3 | F– fhuA53 dapD8 lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 (=supF58) gyrA29 (NalR) Δ(thyA)57 hsdS3(rK– mK–) |
| ED8654 (CGSC) | 11 | F– e14– (McrA.) lac-3 or lacY1 galK2 galT22glnV44supF58metB1hsdR514 ((rK– mK+) trpR55 |
| ED8767 (CGSC) | 6, 11 | F– e14– (McrA.) lac-3 or lacY1 galK2 galT22 glnV44 supF58 metB1 mcrB1 hsdS3 (rK– mK–) |
| GM48 (A) | 12 | F– thr leu thi lacY galK galT ara fhuA tsx dam dcm glnV44 |
| GM2929 (CGSC) | 12 | F– ara-14 leuB6 thi-1 fhuA31 lacY1 tsx-78 galK2galT22glnV44hisG4rpsL136 (StrR) xyl-5 mtl-1 dam13::Tn9 (CamR) dcm-6 mcrB1 hsdR2 ((rK– mK+) mcrA recF143 |
| HB101 (A) | 3, 13 | F–Δ(gpt-proA)62leuB6glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (StrR) xyl-5 mtl-1 recA13 thi-1 |
| JM83 (A) | 2 | F– ara Δ(lac-proAB) rpsL (StrR)[φ80 dlacΔ(lacZ)M15] thi |
| JM103 (A) | 2 | F´ traD36 lacIqΔ(lacZ)M15 proA+B+/ endA1 glnV sbcBC thi-1 rpsL (StrR)Δ(lac-pro) (P1) (rK +mK + rP1 +mP1 +) |
| JM105 (A) | 2 | F´traD36 lacIqΔ(lacZ)M15 proA+B+/thi rpsL (StrR) endA sbcB15 sbcC? hsdR4 (rK– mK+) Δ(lac-proAB) |
| JM110 (A) | 2 | F´traD36 lacIqΔ(lacZ)M15 proA+B+/rpsL (StrR) thr leu thi laY galK galT ara fhuA dam dcm glnV44 Δ(lac-proAB) |
| K802 (A, CGSC) | 3, 6, 8 | See WA802 |
| K803 (A, CGSC) | 3, 6, 8 | See WA803 |
| LE392 (A, CGSC) | 3 | F–e14–(McrA–) hsdR514 (rK– mK+) glnV44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55 |
| MC1061 (A) | 1, 7 | F– araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (StrR) hsdR2 (rK– mK+) mcrA mcrB1 |
| MC4100 (CGSC) | 14 | F– araD139 Δ(argF-lac)U169 rpsL150 (StrR)relA1 flbB5301 deoC1 ptsF25 rbsR |
| MM294 (A) | 8 | F–endA1 hsdR17 (rK– mK+) glnV44 thi-1 relA1? rfbD1? spoT1? |
| NM477 | 5, 6 | C600Δ(hsdMS-mcrB)5 (rK– mK– McrBC–) |
| NM554 | 5 | MC1061 recA13 |
| NM621 | 10 | F– hsdR (rK– mK+) mcrA mcrB glnV44 recD1009 |
| P2392 (S) | 7 | LE392(P2) |
| Q358 (A) | 7 | F– hsdR (rK– mK+) glnV fhuA (φ80r) |
| Q359 (A) | 7 | Q358 (P2 ) |
| RR1 (A) | 3 | HB101 recA+ |
| WA802 (A, CGSC) | 3, 6, 8 | F– e14–(McrA–) lacY1 or Δ(lac)6 glnV44 galK2 galT22 rfbD1 metB1 mcrB1 hsdR2 (rK– mK+) |
| WA803 (A, CGSC) | 3, 6, 8 | F–e14–(McrA–) lacY1 or Δ(lac)6glnV44 galK2 galT22 rfbD1 metB1 mcrB1 hsdS3 (rK– mK–) |
| χ1776 (A, CGSC) | 3 | F– fhuA53 dapD8 minA1 glnV44 (=glnV44) Δ(gal-uvrB)40 minB2 rfb-2 gyrA25 (NalR) thyA142 oms-2metC65 oms-1 (tte-1) Δ(bioH-asd)29 cycB2 cycA1 hsdR2 (rK– mK+) mcrB1? |
| XL1-Blue (S) | 15 | F´ ::Tn10 proA+B+ lacIq Δ(lacZ)M15/ recA1 endA1 gyrA96 (NalR) thi hsdR17 (rK– mK+) glnV44 relA1 lac |
| Y1088 (A) | 4 | F–Δ(lac)U169 glnV supF hsdR (rK– mK+) metB trpR fhuA21 proC::Tn5 (pMC9; TetR AmpR) NOTE: pMC9 is pBR322 with lacIq inserted |
| Y1089† (S) | 4, 16 | F– Δ(lac)U169 lon-100 araD139 strA hflA150::Tn10 (pMC9; TetR AmpR) |
| Y1090†† (S) | 4, 16 | F– Δ(lac)U169 lon-100 araD139 rpsL(StrR) supF mcrA trpC22::Tn10 (pMC9; TetR AmpR) |
† C600 lines obtained from different sources give different results. The original strain and that obtained from the E. coli Genetic Stock Center (Yale University) are McrA+; derivatives traceable to the Brenner laboratory are McrA– (18).
†† No isolates of these strains tested showed pleiotropic phenotypes attributed to lon (i.e. were not mucoid, formed turbid λ plaques, grew well on rich media
The designation R(...) means Rearrangement by analogy with IN(...) for INversion (19). Thus, "R(xxx::Tn10--TetS)" signifies the former presence of a Tn10 insertion. For insertions of wild-type Tn10, the rearrangement was presumably Tn10 promoted, since 99% of TetS derivatives of Tn10-containing strains have acquired transposon-promoted rearrangements (20, 21, 22) that spare one or both IS10 elements.
References
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