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PURExpress^® vs. NEBExpress^® Application Chart

PURExpress^® In Vitro Protein Synthesis Kit (NEB #E6800) and NEBExpress^® Cell-free E. coli Protein Synthesis System (NEB #E5360) are complimentary products that can be used for many of the same applications. However, certain applications may favor one system over the other depending on experimental strategy

	PURExpress In Vitro Protein Synthesis Kit (NEB #E6800)	NEBExpress Cell-free E. coli Protein Synthesis System (NEB #E5360)
Protein Synthesis and Characterization
Toxic proteins	✔	✔
Protease sensitive proteins	✔	✔
Membrane proteins	✔	✔
Antibody fragments	✔	✔
Protein-Protein, Protein-DNA, Protein-RNA interactions & arrays	✔	✔
Direct enzymatic functional assays	✔	✔
Co-expression of multiple target proteins	✔	✔
Protein Engineering
Directed protein evolution including ribosome and mRNA display	✔
High-throughput screening	✔	✔
Functional Genomics or Proteomics Arrays	✔	✔
Protein Labeling and Tagging
Fluorescent labeling of proteins	✔
Radiolabeling	✔
His-tagged proteins		✔
Small affinity tags (i.e., FLAG, strep, StrepII, myc, HA)	✔	✔
Translation and Ribosome Studies
Translational machinery studies	✔
Chaperone and protein folding studies	✔
Synthetic Biology
Metabolic engineering prototyping	✔	✔
Gene circuit studies	✔	✔
Non-natural amino acid incorporation	✔
Other
Diagnostics	✔	✔
Diagnostics Antimicrobial studies or drug screening	✔	✔
Scale-up		✔

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