Properties of DNase Enzymes

Understand the properties of the various DNase enzymes available from NEB.

¹ We do not recommend using Duplex DNase in the presence of calcium (Ca²⁺) as this will increase the enzyme’s promiscuity (activity toward ssDNA).
² We do not recommend using NEBExpress Salt-Active Nuclease with SDS or phosphate buffers. Additionally, we do not recommend substituting Ca²⁺ for Mg²⁺ or Mn²⁺.
³  NEBExpress Salt-Active Nuclease will degrade both the DNA and RNA strand of a DNA: RNA hybrid duplex.
⁴ Heat inactivate Duplex DNase at 75°C for 10 minutes in the presence of 1 mM DTT. If the sample contains RNA, we recommend adding EDTA (10 mM final concentration) and DTT (1 mM final concentration), prior to heat inactivation. RNA may degrade at temperatures > 65°C in the presence of divalent metals such as Mg²⁺.
⁵ NEBExpress Salt Active Nuclease can be heat inactivated by the addition of ≥10 mM EDTA or incubation with 1 mM DTT at 55°C for 30 min, or incubation for 1h at 65°C or 20 min at 95°C. SAN also be removed from a reaction by capture with a cation exchange resin.