NEB Scientific Posters
Your search returned 101 results.
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Rapid, robust and sensitive DNA, RNA and small RNA library construction methods for evaluation of various samples and species
Streamlined NEBNext workflows empower researchers to efficiently generate high-quality sequencing libraries across diverse plant and animal sample types. By combining speed, sensitivity, and consistency, as well as compatibility with automation, they offer a powerful solution for accelerating genomic studies in agricultural research. NEBNext UltraExpress® DNA, NEBNext UltraExpress® FS DNA and NEBNext UltraExpress® RNA library prep kits provide rapid (2-3 hours) and reliable library construction, utilizing a single protocol for all input amounts (10 ng - 200 ng DNA and 25 ng - 250 ng RNA).
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Real-world applications powered by NEBNext® Poster
This attractive poster presents the range of applications for next-generation sequencing in the modern world.
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Detection of Tumor-specific Mutations in Plasma Utilizing an Optimized Cell-free DNA Extraction Workflow
Plasma cell-free DNA (cfDNA) analysis is advancing liquid biopsy for early disease detection, monitoring, and therapy selection, offering improved patient follow-up and cost efficiency over tissue biopsies. Technical challenges persist, including sample stabilization, low cfDNA concentrations, and extraction sensitivity. Monarch magnetic bead-based extraction enables efficient, reproducible isolation of high-quality cfDNA, recovering fragments as small as 50 bp and eluting in low volumes, ready for sequencing and digital PCR. Libraries prepared with NEBNext® Ultra II DNA Library Prep Kit show high conversion efficiency and sequencing quality, supporting sensitive variant detection. Duplex digital PCR assays using extracted cfDNA yield robust, accurate allele frequency quantitation for mutations such as PIK3CA p.E545K. The streamlined workflow integrates seamlessly with NEB downstream solutions, enabling reliable mutation detection from plasma samples and supporting multiple downstream applications.
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High-throughput methyl-binding domain-based DNA enrichment and sequencing
High-throughput methylated DNA enrichment can be an important tool to support high-throughput and large-scale epigenetic studies. Using a fused methyl-CpG binding domain from human MBD2 to the Fc tail of human IgG1 (MBD2-Fc) coupled with paramagnetic hydrophilic protein A beads, methylated DNA can be enriched from mixed genome samples and cell-free DNA (cfDNA) upstream of sequencing.
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Integration of whole exome sequencing and RNA-seq facilitates somatic mutation detection in tumor tissue samples
Somatic mutations are critical targets for disease diagnosis, prognosis, and drug discovery. Whole genome sequencing and targeted sequencing approaches are crucial for identifying somatic mutations in cancer. Meanwhile, RNA-seq has emerged as a complementary tool for somatic mutation profiling, especially for variants of uncertain significance (VUS), to characterize cancer types for precision medicine. Therefore, a comparative study to determine the concordance between DNA-seq and different RNA-seq methods and data analysis tools will help define the optimal approaches for somatic variant detection. This poster presents data from paired tumor and normal tissue samples from rectum and colon cancer patients, analyzing CNVs, SNVs, and indels.
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Automated Protein Expression and Purification of NEBExpress® Cell-free E. coli Protein Synthesis Reactions using NEBExpress Ni-NTA Magnetic Beads
We detail a workflow incorporating a magnetic particle processor, NEBExpress Ni-NTA magnetic beads, and NEBExpress Cell-free E. coli Protein Synthesis reactions that enables users to go from DNA to purified proteins in a single day with less hands-on time and greater reproducibility.
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Cell-Free Production of Antibody Fragments for High-Throughput Analyses
Single-chain antigen-binding fragments (e.g. VHH, scFv) offer several advantages over traditional therapeutic antibodies, including smaller size, enhanced solubility and thermal stability. Here we demonstrate the utility of our cell-free protein synthesis (CFPS) platform, NEBExpress® Cell-free E. coli Protein Synthesis (lysate), for the rapid production of antibody fragments in quantities suitable for direct analysis or further purification. CFPS workflows are fast, scalable and cost-efficient, and can be seamlessly integrated with our high-throughput DNA assembly methods to enable the rapid screening of diverse binder libraries. Additionally, the defined nature of these systems allows for straightforward optimization to improve expression of challenging constructs.
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EnGen® SpRY Cas9 – A universal tool for DNA cleavage in vitro
We demonstrate a simple workflow for universal in vitro DNA cleavage in cloning applications, called SpRYgest, using EnGen® sgRNA synthesis followed by in vitro cleavage with EnGen® SpRY Cas9 RNPs. We confirm that EnGen® SpRY Cas9 ribonucleoproteins (RNPs) can cleave more than 130 target sequences with various PAMs that are incompatible with wildtype Spy Cas9.
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Rapid DIY Gene Synthesis
Gene synthesis from commercial vendors is typically conducted by using overlap extension PCR from oligos manufactured on microarrays. Here we apply Data-Optimized Assembly Design of Golden-Gate overhangs to rapidly construct genes from oligo pools in three steps and as a result create a workflow that can be employed in any standard molecular biology lab.
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Accurate detection of small non-coding RNAs using NEBNext® Low-bias Small RNA Library Prep Kit
This poster describes a novel, ligation-based small RNA library preparation method that is characterized by reduced bias in addition to increased detection of sncRNAs. Libraries can be made in ~3.5 hours using a streamlined protocol with bead-based size-selections and cleanups. The robustness of this method is demonstrated with its compatibility across a broad input range (0.5 ng – 1,000 ng of Total RNA), as well as with challenging sample types, such as formalin-fixed paraffin-embedded (FFPE) RNA. The NEBNext Low-bias Small RNA Library Prep Kit sets a new standard for seeing small RNAs clearly.
