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Total RNA sequencing at single-cell and spatial resolution

Single-cell sequencing has traditionally focused on profiling protein-coding mRNAs and their differential expression. However, this approach overlooks a substantial portion of transcriptome—such as miRNAs, snRNAs, tRNAs, and lncRNAs—which exhibit cell-type specificity and play critical roles in tissue development and disease progression. The Kim lab investigates novel and user-friendly single-cell RNA-seq methods to allow for the analysis of these diverse RNAs species. We also investigate spatial transcriptomics technologies to map total RNAs within tissue, complementing single-cell data with spatial context.