HomeFAQsWhat is the temperature range for MB-CasFire (NEB #M0670) in LAMP?
FAQ: What is the temperature range for MB-CasFire (NEB #M0670) in LAMP?
The one-pot DETECTR® LAMP assay can be performed between 50–65°C. We recommend running a temperature gradient to identify the optimal temperature for enhancing MB-CasFire specificity, especially when detecting single nucleotide polymorphisms (SNPs) in the target sequence.
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