HomeFAQsWhat is the plasmid composition in the Control Template and Primer Mix (S0534AVIAL)?
FAQ: What is the plasmid composition in the Control Template and Primer Mix (S0534AVIAL)?
The two plasmids in the Control Template and Primer Mix are a standard pUC19 plasmid and a pUC19 plasmid with a 10-bp insertion. The ratio of unmodified plasmid to modified (10-bp insertion) plasmid in the control is 4:1. Approximately 20-25% calculated editing efficiency is expected after digestion of the control reaction.
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