Home FAQs What is the lowest elution volume I can use when using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)?

FAQ: What is the lowest elution volume I can use when using the Monarch® Mag Cell-free DNA (cfDNA) Extraction Kit (NEB #T4070)?

Optimal elution volume depends on the sample volume and the magnetic beads. For ideal recovery, the elution volume should be half of the volume of beads used although a lower elution volume, for example, one third of the bead volume used, also produces good recovery. Other factors to consider when choosing the elution buffer volume include the expected cfDNA content in the sample and the downstream application's input requirements.