Home FAQs What are the differences between Exo-CIP Rapid PCR Cleanup Mix (NEB #M1058) and the Exo-CIP Rapid PCR Cleanup Kit (NEB #E1050)?

FAQ: What are the differences between Exo-CIP Rapid PCR Cleanup Mix (NEB #M1058) and the Exo-CIP Rapid PCR Cleanup Kit (NEB #E1050)?

The Exo-CIP Rapid PCR Cleanup Mix (NEB #M1058) is a single-tube enzyme blend containing thermolabile Exonuclease I and thermolabile Calf Intestinal Phosphatase (CIP) in an optimized formulation designed for performance and stability. Instead of adding 1 µl each from Exo-CIP Tube A and Tube B in Exo-CIP Rapid PCR Cleanup Kit (NEB #E1050), customers add 2 µl of Exo-CIP Rapid PCR Cleanup Mix, which streamlines reaction setup and supports automated, high-throughput workflows by eliminating one pipetting step. As with NEB #E1050, the cleaned PCR product is ready in 5 minutes for downstream applications such as Sanger sequencing, SNP detection, or NGS library preparation.