HomeFAQsShould I dialyze my reaction prior to cleavage with Cold-Active TEV Protease (NEB #P8118)?
FAQ: Should I dialyze my reaction prior to cleavage with Cold-Active TEV Protease (NEB #P8118)?
We recommend dialyzing the fusion protein if the buffer has > 2 M urea, > 1 M Guanidine hydrochloride, > 30% glycerol, pH values below 6 or above 9, or cysteine protease inhibitors.
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