HomeFAQsI do not want to use the provided Thymine-DNA Glycosylase (TDG) reaction buffer for my experiment. Do you have any suggestions on how I can optimize my buffer conditions?
FAQ: I do not want to use the provided Thymine-DNA Glycosylase (TDG) reaction buffer for my experiment. Do you have any suggestions on how I can optimize my buffer conditions?
Thymine-DNA Glycosylase (NEB #M0766) works best in the presence of a reducing agent, so the addition of 1 mM fresh DTT or TCEP will greatly improve performance. In the presence of fresh reducing agent, TDG activity can tolerate up to 200 mM NaCl before activity suffers.
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