Yes, Bst-XT WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) is formulated with a mixture of dTTP and dUTP. This ensures both efficient isothermal amplification as well as the incorporation of dU into the reaction products. The mix also contains Antarctic Thermolabile UDG (NEB #M0372). In subsequent reactions, LAMP products containing dU serve as a substrate for UDG, uracil DNA glycosylase, allowing carryover contamination prevention. UDG activity during setup will quickly and efficiently destroy any contaminating products. Because LAMP can generate large quantities of DNA in very short periods of time, best practices to reduce contamination involve avoiding opening LAMP reactions after amplification.
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